S was subject to normalization with median centering and interquartile range (IQR) scaling. The resulting data show equal distribution among various groups of samples. White bar represents samples obtained in the light cycle and black bar for those in the dark cycle.Nature. Author manuscript; readily available in PMC 2014 August 22.Liu et al.PageAuthor Manuscript Author ManuscriptExtended Information Figure six.Flow chart of metabolomics data analysis (displaying the constructive mode metabolites; see solutions for detailed description).Extended Information TableAuthor Manuscript Author ManuscriptList of metabolites recognized by Metaboanalyst system and subsequently used for MSEA analysis.Metabolites 1-Acylglycerol eicosatrienoic acid eicosadienoic acid eicosenoic acid Glycerophosphocholine LysoPC(18:0) LysoPC(18:two) LysoPC(18:3) LysoPC(22:0) LysoPC(22:six) LysoSM(d18:1) HMDB ID METPA0229 HMDB02925 HMDB05060 HMDB02231 HMDB00086 HMDB10384 HMDB10386 HMDB10387 HMDB10398 HMDB10404 HMDBNature. Author manuscript; offered in PMC 2014 August 22.Liu et al.PageAuthor Manuscript Author ManuscriptIMetabolites PA(34:1) hexadecenoic acid Computer(36:1) Pc(32:0) Computer(36:2) PE(34:1) PE(34:2) PE(40:6) PhosphatidylgIyceroI glycerophosphoethanolamine phosphatidylserine PS(36:1) Sphingomyelin sphingosine Sphingosine 1-phosphateHMDB ID HMDB07858 HMDB03229 HMDB07887 HMDB00564 HMDB00593 MSEA0160 MSEA0161 MSEA0170 MSEA0606 MSEA0611 HMDB12330 HMDB10163 HMDB01348 HMDB00252 HMDBExtended Information TableMetabolic traits of mouse models: (I) wt and LPPARDKO mice beneath typical chow eating plan (male, eight?0 weeks old, n=6); (II) vehicle (n=4) and Computer(18:0/18:1) (n=5) treated FVB/NJ-db/db mice.7-Bromo-2-methyloxazolo[4,5-c]pyridine uses Author Manuscript Author Manuscript*C57BL/6J Body Weight (8 wks) (g) Fasting Insulin (ng/mL) Fasting Blood Glucose (mg/dL) ALT (Unit/L) AST (Unit/L) II FVB/NJ-db/db Body Weight (g) Fasting Insulin (ng/mL) Fasting Blood Glucose (mg/dL)WT 26.2-Methyl-5-nitropyridin-3-amine Chemical name 03?.PMID:35850484 67 0.35?.10 83.13?.63 25.19?.48 102.70?5.LPPARDKO 24.63?.23 0.31?.05 79.13?.66 18.23?.66 96.78?five.Manage 37.70?.97 36.70?.96 245.7?.Computer(18:0/18:1) 35.98?.94 42.61?.12 184.two?3.20*p0.05, two-tailed t-test. Information have been presented as mean EM.Nature. Author manuscript; offered in PMC 2014 August 22.Liu et al.PageAuthor Manuscript Author Manuscript Author Manuscript Author ManuscriptExtended Information TableList of animal cohorts applied for this study. ED: Extended Data.Cohort Name No. Cohorts Age Sex Background Animal No. Figures Fig. 1a,b; Fig. 3c,d; Fig. 4a; ED. Fig 1a; ED. Fig 2f; ED. Fig. 3e Fig. 1c ; Fig. 3c,d; ED. Fig 3cadPPAR vs adGFP8?0 weeksMaleC57BL/6J4?/genotypeLACC1KD vs Scramble LPPARDKO vs wt, chow fed, metabolic phenotyping8?0 weeks 8?0 and 32?35 weeksMaleC57BL/6J5/genotypeMaleC57BL/6J6?/genotypeED. Fig. 2d Fig. 2a,c ; Fig. 3a ; Fig. 4b; ED. Fig. 2a; ED. Fig. 3c Fig. 2b; ED. Fig. 2c; ED. Fig. 3a,b,f; ED. Fig. 4a Fig. 3f; Fig. 4c; ED. Fig. 4b ED. Fig. 3h Fig. 3g Fig. 3i; Fig. 4d Fig. 4g ; ED. Fig. 4hLPPARDKO vs wt, ad lib circadian study LPPARDKO vs wt, daytime restricted feeding LPPARDKO vs wt. GW501516 treatment wt, Pc intra-peritoneal injection wt, Pc tall vein injection PPAR KO vs wt, lipid infusion wt, db/db, Computer (18:0/18:1) vs vehicle wt, C57BL/5.1, Chow vs Higher Fat8 weeksMale /FemaleC57BL/6J3?/genotype/time point8 weeksMaleC57BL/6J3/genotype/time point four?5/genotype/treatme nt 4/genotype/treatme nt 6/genotype/treatme nt 67/genotype/treatme nt1 110?two weeks 10?two weeks 8?0 weeksMale Male MaleC57BL/6J C57BL/6J FVB/NJ10?2 weeksMaleC57BL/6J8 weeksMaleFVB/NJ4?/treatment 3?/treatment/t.