Gazine | July-September 2014 | Vol 10 | IssueZhu, et al.: Qualitative and quantitative analysis of constituents in Danmu injectionA10ODglucopyranoside (D15), naucleamide G (D17) and pumiloside (D18) are larger content material, while other six compounds have been located to be reduced in Danmu injection.and quantitative information and facts for analysis of chemical constituents in Danmu injection. Twentyfive compoundsCONCLUSIONChemical constituents of Danmu injection was systematically and completely investigated by HPLC?DAD SIMSn, which provided fullscale qualitativeFigure five: MS2, MS3 spectrum of loganinFigure 7: MS/MS, MS3and MS4 spectrum of pumiloside and 3-epipumilosideFigure 6: Fragmentations pathways of naucleamide GPharmacognosy Magazine | July-September 2014 | Vol ten | Challenge 39Zhu, et al.: Qualitative and quantitative analysis of constituents in Danmu injectionFigure 8: MS/MS, MS3,MS4,MS5 spectrum of strictosamide and vincosamideNH N H+O N HNH+ONH N H+OHH-gluOHH-H2OOHHOm/zO HO OH OH OH ORDAEm/zOHRD A-Cm/zRAD-DNH N H+NH N H+RDA-CHN H+OHm/zm/zm/zO N+N H H+N N+m/zHm/zm/zFigure 9: Fragmentations pathways of strictosamide and vincosamideincluding phenolic acid, phenol glycoside, iridous glycoside and alkaloid glycoside have been identified; eight (D3, D5, D6, D8, D9, D11, D17 and D23) of them have been in no way reported previously in Nauclea officinalis.The quantitative process was proved to have exceptional linearity, excellent accuracy, sensitivity and repeatability. The results would supply the chemical help for the further pharmacokinetic research and for powerful quality assessment of DanmuPharmacognosy Magazine | July-September 2014 | Vol 10 | IssueZhu, et al.: Qualitative and quantitative evaluation of constituents in Danmu injectionFigure ten: Fragmentations pathways of three,5-tetrahydrodeoxycordifoline lactamTable three: Precisions and recoveries of 11 analyses (110801)Analyte D1 D3 D6 D9 D15 D17 D18 D19 D23 D24 D25 Intra-day (n=6) ( /mL) Means .D 26.36?.36 11.42?.17 9.40?.14 7.55?.12 20.02?.26 16.81?.24 38.64?.54 19.11?.42 eight.69?.21 123.89?.96 three.96?.052 RSD ( ) 1.37 1.49 1.49 1.59 1.30 1.43 1.40 two.20 2.42 1.58 1.31 Inter-day (n=3) ( /mL) Means .D 26.08?.44 11.09?.30 9.61?.11 7.45?.21 20.17?.43 16.26?.37 38.94?.70 20.83?.43 7.99?.22 125.53?.25 four.05?.062 RSD ( ) 1.69 two.71 1.14 two.82 two.13 two.28 1.80 2.06 2.75 1.79 1.53 Initial ( ) 263.60 114.20 94.00 75.50 200.20 168.10 386.40 191.ten 86.90 1238.90 39.60 Added ( ) 266.11 113.76 95.52 73.92 194.40 169.12 386.91 192.00 84.40 1202.40 39.36 Recoveries (n=6) Found ( ) 542.91 223.39 191.45 148.32 389.70 352.95 775.82 391.34 174.33 2428.80 80.44 Recovery ( ) 104.96 95.97 102.02 98.61 97.48 109.30 100.65 104.29 103.59 98.96 103.77 RSD ( ) 4.23 two.53 three.36 4.07 three.78 2.27 3.45 1.79 2.07 1.64 1.RSD: Relative standard deviation; S.D: Typical deviationTable four: The content material of 11 compounds in 5 batches of Danmu injection (n=3, /mL)Analyte D1 D3 D6 D9 D15 D17 D18 D19 D23 D24 D25 110801 259.Cholic acid site 65 115.6-Bromo-2H-benzofuran-3-one uses 25 96.PMID:23724934 97 75.75 199.28 166.18 390.41 196.12 83.25 1240.91 40.66 110601 254.62 82.81 20.39 76.02 289.51 489.55 177.47 20.75 120.91 800.54 21.81 110616 239.64 37.71 21.04 22.95 157.18 74.83 142.84 15.32 47.04 1294.15 56.57 110401 353.91 one hundred.57 83.62 96.77 237.55 356.92 193.94 73.94 62.55 789.57 39.65 110925 423.87 106.55 84.57 65.91 168.34 102.2 126.85 18.95 26.35 1080.43 25.injection, which would be of important value for the safety use and modern day study of Danmu injection.ACKNOWLEDGEMENTSThe analysis was financially supported by the National Natur.