Soil, and soil traits such as organic matter content material, pH values, and phosphorous concentrations. IAA and GA3 production in our collection of Azotobacter strains was greater than that reported for any phyllospheric A. chroococcum strain REN2 [9]. Conversely, other Azotobacter strains, isolated from rhizospheric soil in India, reached the same IAA production levels than our highIAAproducing strains [29]. While all tested strains excreted phytohormones in chemical complex expanding medium, the levels of IAA, GA3 , and Z production differed among them. Interestingly, IAA production showed higher levels in nearly all A. chroococcum strains but variable levels inside a. salinestris strains, agreeing with its larger intraspecific diversity revealed by repPCR. Although the production of phytohormones by5. ConclusionsThe genotyping of azotobacterial isolates by the combined analysis of ARDRA and repPCR and also the screening of isolates based on their in vitro traits for prospective plant development advertising activity had been valuable tools for their taxonomic classification and phenotypic characterization.DMT-2’fluoro-da(bz) amidite Price This survey, embracing diverse regions of Argentina, allowed us to have a 1st strategy to the presence of this bacterial genus in soils. Evaluation of plant growthpromoting traits in bacterial strains is actually a crucial activity as criteria for strain selection for biofertilizer formulations. As biofertilizers are a complex resulting from bacteria and their metabolites excreted to the growing medium, it becomes relevant to evaluate each constituent of a biofertilizer prior to contemplating it as a prospective candidate for field application. Thus, our results constitute a crucial technological contribution to Azotobacter strain selection for biofertilizer formulations that would aid to implement a additional sustainable agriculture by means of decreasing the usage of agrochemicals.Conflict of InterestsThe authors declare that there is no conflict of interests with regards to the publication of this paper.AcknowledgmentsThe authors thank the Instituto Nacional de Tecnolog i Agropecuaria (INTA), the Instituto de Investigaciones en Biociencias Agr olas y Ambientales (INBACONICET/ i UBA), and C edra de Microbiolog Agr ola, Facultad de a i i Agronom , Universidad de Buenos Aires, for their help i to carry out this research.The Scientific World Journal[16] S. F. Altschul, T. L. Madden, A. A. Sch�ffer et al., “Gapped a BLAST and PSIBLAST: a brand new generation of protein database search programs,” Nucleic Acids Investigation, vol. 25, no. 17, pp. 3389402, 1997. [17] S. P ezMiranda, N. Cabirol, R. GeorgeT lez, L. S. Zamudioe e Rivera, and F. J. Fern dez, “OCAS, a rapidly and universal a method for siderophore detection,” Journal of Microbiological Strategies, vol.2-Amino-2-methyl-1-propanol custom synthesis 70, no.PMID:24025603 1, pp. 12731, 2007. [18] R. I. Pikovskaya, “Mobilization of phosphorus in soil in connection with vital activity of some microbial species,” Microbiologiya, vol. 17, pp. 36270, 1948. [19] C. S. Nautiyal, “An efficient microbiological development medium for screening phosphate solubilizing microorganisms,” FEMS Microbiology Letters, vol. 170, no. 1, pp. 26570, 1999. [20] E. Glickmann and Y. Dessaux, “A crucial examination from the specificity with the Salkowski reagent for indolic compounds created by phytopathogenic bacteria,” Applied and Environmental Microbiology, vol. 61, no. 2, pp. 79396, 1995. [21] D. Perrig, M. L. Boiero, O. A. Masciarelli et al., “Plantgrowthpromoting compounds created by two agronomically essential strain.